Basic Concepts in Digital Image Processing
Digital image processing enables virtually noise-free modification of an image in the form of a matrix of integers instead of the classical darkroom manipulations necessary for analog images and video signals.
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Anatomy of the Fluorescence Microscope
Fluorescence microscopes have evolved with speed over the past decade, coupled to equally rapid advances in laser technology, solid-state detectors, interference thin film fabrication, and computer-based image analysis.
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Basic Concepts in Fluorescence
When coupled to the optical microscope, fluorescence enables investigators to study a phenomena in cellular biology. Foremost is the analysis of intracellular distribution of specific macromolecules in sub-cellular assemblies.
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Hoffman Modulation Contrast Basics
The featured section serves as an introduction to the basic concepts in modulation contrast microscopy with numerous illustrations and examples of how the technique is implemented.
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Fluorescence and DIC Combination Microscopy
Fluorescence microscopy can be combined with contrast enhancing techniques, such as differential interference contrast (DIC) and phase contrast illumination, to minimize the effects of photobleaching.
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Comparison of Phase Contrast and DIC Microscopy
The most fundamental distinction between differential interference contrast and phase contrast microscopy is the optical basis upon which images are formed.
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DIC Microscope Configuration and Alignment
DIC components can be installed on virtually any brightfield transmitted, reflected, or inverted microscope, provided the instrument is able to accept polarizing filters and the specially designed condenser and objective prisms.
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Fundamental Concepts in DIC Microscopy
Through a mechanism different from phase contrast, differential interference contrast converts specimen optical path gradients in o amplitude differences that can be visualized as improved contrast in the resulting image.
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