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Anatomy of the Fluorescence Microscope
Fluorescence microscopes have evolved with speed over the past decade, coupled to equally rapid advances in laser technology, solid-state detectors, interference thin film fabrication, and computer-based image analysis.
2018-04-25 Pooher Inc. 559
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Light Sources
Inordertogenerateenoughexcitationlightintensitytofurnishsecondaryfluorescenceemissioncapableofdetection,powerfullightsourcesareneededsuchasmercuryorxenonarc(burner)lamps.
2018-04-25 Pooher Inc. 228
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Reflected Darkfield Illumination
One of the most effective ways to improve contrast in the reflected light microscope is to utilize darkfield illumination. In reflected darkfield microscopy, an opaque occluding disk is placed in the path of the light traveling through the vertical illuminator so that only the peripheral rays of light reach the deflecting mirror.
2018-04-17 Pooher Inc. 306
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Reflected Light Microscopy
Reflected light microscopy is often referred to as incident light, epi-illumination, or metallurgical microscopy, and is the method of choice for fluorescence and for imaging specimens that remain opaque even when ground to a thickness of 30 microns.
2018-04-13 Pooher Inc. 208
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Anatomy of a Microscope - Image Brightness
Regardless of the imaging mode utilized in optical microscopy, image brightness is governed by the light-gathering power of the objective, which is a function of numerical aperture.
2018-03-27 Pooher Inc. 243
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Köhler Illumination - Köhler Illumination in Transmitted Light
Adjustment of a transmitted light microscope for Köhler illumination is a logical and relatively easy process that should be practiced until mastered by all serious students of microscopy.
2018-03-26 Pooher Inc. 226
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Köhler Illumination - Light Sources
Early microscopists relied on oil lamps and natural sunlight to provide an external source of illumination for their primitive (but often remarkably accurate) microscopes.
2018-03-26 Pooher Inc. 128
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Microscope Illumination
Microscope Illumination
2018-03-22 Pooher Inc. 205
