• Introduction to CMOS Image Sensors

    CMOS image sensors are designed with the ability to integrate a number of processing and control functions, which lie beyond the primary task of photon collection, directly onto the sensor integrated circuit.

    2018-04-26 Pooher Inc. 247

  • Basic Concepts in Digital Image Processing

    Digital image processing enables virtually noise-free modification of an image in the form of a matrix of integers instead of the classical darkroom manipulations necessary for analog images and video signals.

    2018-04-26 Pooher Inc. 684

  • Anatomy of the Fluorescence Microscope

    Fluorescence microscopes have evolved with speed over the past decade, coupled to equally rapid advances in laser technology, solid-state detectors, interference thin film fabrication, and computer-based image analysis.

    2018-04-25 Pooher Inc. 354

  • Basic Concepts in Fluorescence

    When coupled to the optical microscope, fluorescence enables investigators to study a phenomena in cellular biology. Foremost is the analysis of intracellular distribution of specific macromolecules in sub-cellular assemblies.

    2018-04-25 Pooher Inc. 53

  • Brief Overview of Fluorescence Filters

    A wide spectrum of filter cubes is available from most major manufacturers, which now produce filter sets capable of imaging most of the common chromophores in use today.

    2018-04-25 Pooher Inc. 333

  • Hoffman Modulation Contrast Basics

    The featured section serves as an introduction to the basic concepts in modulation contrast microscopy with numerous illustrations and examples of how the technique is implemented.

    2018-04-19 Pooher Inc. 55

  • DIC Microscope Configuration and Alignment

    DIC components can be installed on virtually any brightfield transmitted, reflected, or inverted microscope, provided the instrument is able to accept polarizing filters and the specially designed condenser and objective prisms.

    2018-04-18 Pooher Inc. 93

  • Contrast in Optical Microscopy

    When imaging specimens in the optical microscope, differences in intensity and/or color create image contrast, which allows individual features and details of the specimen to become visible. Contrast is defined as the difference in light intensity between the image and the adjacent background relative to the overall background intensity. In general, a minimum contrast value of 0.02 (2 percent) is needed by the human eye to distinguish differences between the image and its background.

    2018-04-16 Pooher Inc. 76

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