• Basic Concepts in Digital Image Processing

    Digital image processing enables virtually noise-free modification of an image in the form of a matrix of integers instead of the classical darkroom manipulations necessary for analog images and video signals.

    2018-04-26 Pooher Inc. 732

  • Anatomy of the Fluorescence Microscope

    Fluorescence microscopes have evolved with speed over the past decade, coupled to equally rapid advances in laser technology, solid-state detectors, interference thin film fabrication, and computer-based image analysis.

    2018-04-25 Pooher Inc. 414

  • Hoffman Modulation Contrast Basics

    The featured section serves as an introduction to the basic concepts in modulation contrast microscopy with numerous illustrations and examples of how the technique is implemented.

    2018-04-19 Pooher Inc. 66

  • Fluorescence and DIC Combination Microscopy

    Fluorescence microscopy can be combined with contrast enhancing techniques, such as differential interference contrast (DIC) and phase contrast illumination, to minimize the effects of photobleaching.

    2018-04-18 Pooher Inc. 114

  • DIC Microscope Configuration and Alignment

    DIC components can be installed on virtually any brightfield transmitted, reflected, or inverted microscope, provided the instrument is able to accept polarizing filters and the specially designed condenser and objective prisms.

    2018-04-18 Pooher Inc. 105

  • Fundamental Concepts in DIC Microscopy

    Through a mechanism different from phase contrast, differential interference contrast converts specimen optical path gradients into amplitude differences that can be visualized as improved contrast in the resulting image.

    2018-04-18 Pooher Inc. 99

  • Brief Overview of DIC Microscopy

    In the mid-1950s, a French optics theoretician named Georges Nomarski modified the Wollaston prism used for detecting optical gradients in specimens and converting them into intensity differences.

    2018-04-18 Pooher Inc. 93

  • Reflected Darkfield Illumination

    One of the most effective ways to improve contrast in the reflected light microscope is to utilize darkfield illumination. In reflected darkfield microscopy, an opaque occluding disk is placed in the path of the light traveling through the vertical illuminator so that only the peripheral rays of light reach the deflecting mirror.

    2018-04-17 Pooher Inc. 162

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