• Anatomy of the Fluorescence Microscope

    Fluorescence microscopes have evolved with speed over the past decade, coupled to equally rapid advances in laser technology, solid-state detectors, interference thin film fabrication, and computer-based image analysis.

    2018-04-25 Pooher Inc. 414

  • Basic Concepts in Fluorescence

    When coupled to the optical microscope, fluorescence enables investigators to study a phenomena in cellular biology. Foremost is the analysis of intracellular distribution of specific macromolecules in sub-cellular assemblies.

    2018-04-25 Pooher Inc. 97

  • Hoffman Modulation Contrast Basics

    The featured section serves as an introduction to the basic concepts in modulation contrast microscopy with numerous illustrations and examples of how the technique is implemented.

    2018-04-19 Pooher Inc. 66

  • Fluorescence and DIC Combination Microscopy

    Fluorescence microscopy can be combined with contrast enhancing techniques, such as differential interference contrast (DIC) and phase contrast illumination, to minimize the effects of photobleaching.

    2018-04-18 Pooher Inc. 114

  • Comparison of Phase Contrast and DIC Microscopy

    The most fundamental distinction between differential interference contrast and phase contrast microscopy is the optical basis upon which images are formed.

    2018-04-18 Pooher Inc. 46

  • DIC Microscope Configuration and Alignment

    DIC components can be installed on virtually any brightfield transmitted, reflected, or inverted microscope, provided the instrument is able to accept polarizing filters and the specially designed condenser and objective prisms.

    2018-04-18 Pooher Inc. 105

  • Fundamental Concepts in DIC Microscopy

    Through a mechanism different from phase contrast, differential interference contrast converts specimen optical path gradients into amplitude differences that can be visualized as improved contrast in the resulting image.

    2018-04-18 Pooher Inc. 99

  • Darkfield Microscope Configuration

    The following section reviews the steps in the configuration and alignment of a microscope for both low and high magnification transmitted darkfield illumination.

    2018-04-17 Pooher Inc. 258

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