• Basic Concepts in Digital Image Processing

    Digital image processing enables virtually noise-free modification of an image in the form of a matrix of integers instead of the classical darkroom manipulations necessary for analog images and video signals.

    2018-04-26 Pooher Inc. 732

  • Anatomy of the Fluorescence Microscope

    Fluorescence microscopes have evolved with speed over the past decade, coupled to equally rapid advances in laser technology, solid-state detectors, interference thin film fabrication, and computer-based image analysis.

    2018-04-25 Pooher Inc. 414

  • Light Sources


    2018-04-25 Pooher Inc. 141

  • Hoffman Modulation Contrast Basics

    The featured section serves as an introduction to the basic concepts in modulation contrast microscopy with numerous illustrations and examples of how the technique is implemented.

    2018-04-19 Pooher Inc. 66

  • Comparison of Phase Contrast and DIC Microscopy

    The most fundamental distinction between differential interference contrast and phase contrast microscopy is the optical basis upon which images are formed.

    2018-04-18 Pooher Inc. 46

  • Fundamental Concepts in DIC Microscopy

    Through a mechanism different from phase contrast, differential interference contrast converts specimen optical path gradients into amplitude differences that can be visualized as improved contrast in the resulting image.

    2018-04-18 Pooher Inc. 99

  • Brief Overview of DIC Microscopy

    In the mid-1950s, a French optics theoretician named Georges Nomarski modified the Wollaston prism used for detecting optical gradients in specimens and converting them into intensity differences.

    2018-04-18 Pooher Inc. 93

  • Transmitted Darkfield Illumination


    2018-04-17 Pooher Inc. 147

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