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Anatomy of the Fluorescence Microscope
Fluorescence microscopes have evolved with speed over the past decade, coupled to equally rapid advances in laser technology, solid-state detectors, interference thin film fabrication, and computer-based image analysis.
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Light Sources
Inordertogenerateenoughexcitationlightintensitytofurnishsecondaryfluorescenceemissioncapableofdetection,powerfullightsourcesareneededsuchasmercuryorxenonarc(burner)lamps.
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Basic Concepts in Fluorescence
When coupled to the optical microscope, fluorescence enables investigators to study a phenomena in cellular biology. Foremost is the analysis of intracellular distribution of specific macromolecules in sub-cellular assemblies.
2018-04-25 Pooher Inc. 216
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Hoffman Modulation Contrast Basics
The featured section serves as an introduction to the basic concepts in modulation contrast microscopy with numerous illustrations and examples of how the technique is implemented.
2018-04-19 Pooher Inc. 183
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DIC Microscope Configuration and Alignment
DIC components can be installed on virtually any brightfield transmitted, reflected, or inverted microscope, provided the instrument is able to accept polarizing filters and the specially designed condenser and objective prisms.
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Fundamental Concepts in DIC Microscopy
Through a mechanism different from phase contrast, differential interference contrast converts specimen optical path gradients in o amplitude differences that can be visualized as improved contrast in the resulting image.
2018-04-18 Pooher Inc. 274
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Brief Overview of DIC Microscopy
In the mid-1950s, a French optics theoretician named Georges Nomarski modified the Wollaston prism used for detecting optical gradients in specimens and converting them in o intensity differences.
2018-04-18 Pooher Inc. 196
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Darkfield Microscope Configuration
The following section reviews the steps in the configuration and alignment of a microscope for both low and high magnification transmitted darkfield illumination.
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