• Comparison of Phase Contrast and DIC Microscopy

    The most fundamental distinction between differential interference contrast and phase contrast microscopy is the optical basis upon which images are formed.

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  • DIC Microscope Configuration and Alignment

    DIC components can be installed on virtually any brightfield transmitted, reflected, or inverted microscope, provided the instrument is able to accept polarizing filters and the specially designed condenser and objective prisms.

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  • Fundamental Concepts in DIC Microscopy

    Through a mechanism different from phase contrast, differential interference contrast converts specimen optical path gradients in o amplitude differences that can be visualized as improved contrast in the resulting image.

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  • Brief Overview of DIC Microscopy

    In the mid-1950s, a French optics theoretician named Georges Nomarski modified the Wollaston prism used for detecting optical gradients in specimens and converting them in o intensity differences.

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  • Darkfield Microscope Configuration

    The following section reviews the steps in the configuration and alignment of a microscope for both low and high magnification transmitted darkfield illumination.

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  • Reflected Darkfield Illumination

    One of the most effective ways to improve contrast in the reflected light microscope is to utilize darkfield illumination. In reflected darkfield microscopy, an opaque occluding disk is placed in the path of the light traveling through the vertical illuminator so that only the peripheral rays of light reach the deflecting mirror.

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  • Transmitted Darkfield Illumination

    Allofusarequitefamiliarwiththeappearanceandvisibilityofstarsonadarknight,thisdespitetheirenormousdistancesfromtheEarth.

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  • Contrast in Optical Microscopy

    When imaging specimens in the optical microscope, differences in intensity and/or color create image contrast, which allows individual features and details of the specimen to become visible. Contrast is defined as the difference in light intensity between the image and the adjacent background relative to the overall background intensity. In general, a minimum contrast value of 0.02 (2 percent) is needed by the human eye to distinguish differences between the image and its background.

    2018-04-16 Pooher Inc. 161

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